Composite

Part:BBa_K2738104:Design

Designed by: Darshak Bhatt   Group: iGEM18_Paris_Bettencourt   (2018-10-10)


Enterocin fused to N-ter designed protein lyase cage monomer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The StarCore described here is a result of the fusion of protein-lyase-cage (self-assembling protein) to Enterocin A (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter.


Source

Enterocin is from Enterococcus faecalis P-lyase cage is synthetic protein

References